Emirates will operate a one-off, 15-hour A380 service to Sao Paulo next month.
The Gulf carrier will roster the superjumbo between Dubai and Sao Paulo's Guarulhos International airport on November 14.
It claims the flight will be the "first-ever commercial Airbus A380 flight to Brazil and South America".
Flights EK261/262 are operated by a B777-300ER with the outbound service to Sao Paulo taking 15 hours and 15 minutes, according to Emirates' website.
EK261 departs Dubai at 1015 and arrives in Sao Paulo at 1930. Return leg EK262 leaves Sao Paulo at 0125 the next day and lands in Dubai at 2125.
Emirates flew a one-off A380 service to Taiwan in the summer (see news, July 21).
It is the airline's policy to run proving flights, to see if ground handling at the destination airport can cope, and then to follow-up with a regular scheduled service at some future stage.
Orhan Abbas, Emirates senior vice president, said: "While we don't currently have plans to introduce the A380 on a scheduled basis to Sao Paulo, our Brazilian customers can still experience the Emirates A380 through seamless and convenient connections from our hub Dubai to more than 30 destinations across our network, including the Far East, Australia and New Zealand."