Flybe will suspend its London Southend to Dublin route next month.
The regional carrier will no longer operate the service, which currently flies three-times weekly, down from daily previously, from April 13.
Paul Simmons, Flybe CCO, said: "As part of a recent review, we have taken the decision to suspend this route with effect from April 13 as passenger numbers regretfully continue to remain below those projected.
"Passengers who have booked to fly on this route are being contacted and offered an alternative flight or a full refund. We sincerely apologise for any inconvenience this may cause our customers."
Earlier this month, Flybe announced it will open a base in Cardiff this summer (see news, March 5).