Norwegian will launch a London Gatwick to Orlando International (MCO) service next summer.
The once-weekly route will depart on Saturdays from April 4.
The airline will also fly to Orlando once per week from Copenhagen — each Monday, from March 30.
The announcement sees Norwegian continue to expand its transatlantic operation.
It will take delivery of its eighth 787 Dreamliner next spring as it looks to boost capacity on existing routes.
As previously reported, Norwegian will double its services from Gatwick to both New York JFK and Los Angeles next summer (see news, September 2). It will also reduced servce on six other routes.
Thomas Ramdahl, Norwegian's CCO, said: "We're happy to announce that we will offer Orlando as a new destination from London and Copenhagen.
"Orlando is already a very popular destination being offered from Oslo and we are looking forward to giving even more passengers the chance to visit this great area of Florida."