Atlasjet will launch a daily service between London Luton and Istanbul Ataturk in May.
The Turkey-based airline will deploy a 200-seat A321-200 in a two-class configuration on the route, with connections to Cyprus and Turkey.
The carrier said the daily flights, which start on May 2, will provide "significant" trade and leisure links.
Yuksel Atak, Atlasjet's ground operations director, said: "We are delighted to connect Istanbul to London Luton with our new flights and look forward to creating a long and successful partnership."
Simon Harley, London Luton's business development manager, added: "We look forward to welcoming Atlasjet to the list of airlines that have chosen to operate from London Luton.
"With Atlasjet's quality on-board service and competitive fares, we are sure the Istanbul flights will be a great success."